USDA scientists have developed a new mobile phone line that quickly and accurately detect thefoot - and - mouth diseasevirus , a highly transmissible and economically devastating disease incattleand other bisulcate - hoofed animals .
The cell strain was created by Agricultural Research Service scientists at the Plum Island Animal Disease Center , Orient Point , N.Y. , and the research was published online in theJournal of Clinical Microbiology .
“ The new cells detect the FMD virus in field sample distribution that come like a shot from naturally infect animals quicker than subsist cell line currently used for nosology , ” say Luis Rodriguez , enquiry leader at Plum Island ’s Foreign Animal Disease Research Unit . “ The new cell are the first permanent cell line capable of name all seven serotypes of FMD virus . ”
The United States has not had an irruption of foot - and - mouth disease in more than 80 years , though the disease is still a serious threat and is consider to be the most economically withering livestock disease worldwide . Outbreaks in other countries have resulted in the whipping of millions of animals to preclude the disease ’s cattle ranch . Rapid detection of the virus during outbreaks will allow researchers to cursorily develop the appropriate vaccine among the seven serotypes ( or virus variations ) and dozens of subtypes , thereby saving valuable sentence and 1000000 of dollars in animal yield .
The fresh cell line stems from earliest inquiry where FADRU scientist Hernando Duque isolated a primary mobile phone sensory receptor that allows the human foot - and - mouth disease virus to attach to and enter the animate being ’s cells and double , Rodriguez say . The approach used to make the new cell line lie of cloning the FMD receptor cistron from bovine tissue paper and incorporating them into a cell logical argument antecedently set up at Plum Island , and then comparing them to other cells currently used in diagnosing and study foundation - and - mouth disease .
mental test were design to validate the cadre line by compare it to other cadre types using virus samples from brute tissues . The new cell line of credit prove to be faster and more reliable than all current symptomatic cell production line in notice the virus in infected cows and slovenly person tissue paper samples from numerous nation .
“ Other cell case presently used to diagnose FMD do n’t survive long and have to be obtain directly from animate being as primary cell polish , cause variation from one batch to the other , ” Rodriguez says . “ This young cell line can be continually turn in culture , maintains susceptibility to FMD virus much longer , and does n’t require pay off new cell from animals repeatedly . ”
Scientists have applied for a letters patent on the unexampled cell line and are making plans to distribute it to diagnostic testing ground in the United States and other countries .
